Alveolar macrophages expressed many different C-type lectin receptors, presumably to recognize and engulf inhaled microorganisms. Gosselin D, Glass CK. BMC Immunol 4: Meta-analysis of lineage-specific gene expression signatures in mouse leukocyte populations. Because the stromal component of a tumor biopsy is not uniform, each sample differs in its relative macrophage content.
Each stimulus produced a stereotypical response 77 , but there was also variation in expression of individual genes, with eQTLs associated with the majority of loci. For example, the promoters of these phagocyte-restricted genes contain purine-rich motifs binding sites for the macrophage-specific transcription factor, PU. These high levels permit PU. Unbiased reconstruction of a mammalian transcriptional network mediating pathogen responses. J Exp Med This response involves the sequential induction of hundreds of genes and includes a complex intrinsic and inducible feedback control network [see below, Ref.
The dendritic cell lineage: The quite different response to macrophages to toll-like receptor agonists such as LPS 54 — 59 has also been called an M1 response. The primary data summarized in this image are derived from Arner et al. A critical review of the experimental basis for this conclusion has been published elsewhere 8.
The deluge of complex information related to macrophage activation can be dda with new analytical tools and new databases that provide access for the non-expert. In mRNA from the wall of the gut, macrophage-specific transcripts derived from the abundant lamina propria macrophage population were easily detected, but the C-type lectins were undetectable.
Ronnblom L, Eloranta ML.
Comparison of the expression and function of the transcription factor PU. The nomenclature has become increasingly confused. They include many inducible splice variants that encode competitive inhibitors of signaling TFEC is a macrophage-restricted member of the microphthalmia-TFE subfamily of basic helix-loop-helix leucine zipper transcription factors.
Genes Dev A transcriptional perspective on human macrophage biology. Front Immunol 4: Liu K, Nussenzweig MC. Note that the most abundant TSS, p1 serpinA1, was detected most highly in liver and in primary hepatocyte libraries, and much less in the relatively de-differentiated HepG2 cells. Biochim Biophys Acta 7: The transcriptional network that controls growth arrest and differentiation in plwn human myeloid leukemia cell line.
So, I take the view that antigen presentation is a function, not a cell type, and prefer to restrict the use businesss the term DC to APCs that depend up Flt3L. The same data supported a study of the process of macrophage differentiation from pluripotent progenitors through monocytes to macrophages 40 Innate immune activity conditions the effect of regulatory variants upon monocyte gene expression.
Mucosal Immunol 1: Several of these factors have well-documented repressive roles. At a single cell level, there is essentially bimodal variation; genes are either induced by LPS or they are not Fate mapping analysis reveals that adult microglia derive from primitive macrophages.
Figures 1 and 2 show examples of the power of this data. Medzhitov R, Horng T. No use, distribution or reproduction is permitted which does not comply with these terms. Development of monocytes, macrophages, and dendritic cells. Features of the regulatory cascade are exemplified by the detailed analysis of the response to LPS. The original data were produced in collaboration with Kenneth Baillie.
However, there was a business conservation of individual elements between the species. Gordon S, Martinez FO. The signaling pathway from the LPS receptor, TLR4, leading to transcriptional activation, has been reviewed in detail by others BowdishMcMaster University, Canada.
Transcript dynamics of proinflammatory genes revealed by sequence analysis of subcellular RNA fractions.
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